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Oral swab genomic DNA extraction kit 50T (centrifugal column method)

Oral swab genomic DNA extraction kit 50T (centrifugal column method)

Oral swab genomic DNA extraction kit 50T (centrifugal column method)

Oral swab genomic DNA extraction kit 50T (centrifugal column method)

Product Name: Buccal Swarm Genomic DNA Extraction Kit
Specifications: 50T
Scope of application: Suitable for rapid extraction of genomic DNA from buccal swabs
Product description:
The kit adopts a centrifugal adsorption column and a unique buffer system which can specifically bind DNA. The silicon matrix material used in the centrifugal adsorption column is a unique new material of the company, which can efficiently and specifically adsorb DNA, and can remove impurities and impurities to the utmost extent. Other organic compounds in the cell. The extracted genomic DNA fragments are large, high in purity, and stable in quality. The unique binding solution of this product rapidly lyses cells and inactivates intracellular nucleases. The genomic DNA is then selectively adsorbed onto the glass fiber membrane, and the cells are metabolized by a series of rapid rinsing-separation steps, inhibitor removal and rinsing. Impurities such as substances and proteins are removed, and finally the low salt elution buffer elutes the clean genomic DNA from the membrane.
• Do not use reagents such as toxic phenol, or ethanol precipitation, and eliminate the centrifugation step.
• Quick and easy, a single buccal sample operation can be completed in less than 30 minutes, allowing multiple samples to be run simultaneously.
• Multiple column rinses ensure high purity. The extracted genomic DNA OD260/OD280 has a typical ratio of 1.7-1.9 and a length of 20kb -50kb. It can be directly used in PCR, outward-blot and various digestion reactions.
• The eluent does not contain the chelating agent EDTA and does not affect downstream digestion, ligation and other reactions. It is also possible to use water to elute, but it should be ensured that the pH is greater than 7.5 and the pH is too low to affect the elution efficiency. The DNA eluted with water should be stored at -20 °C. If the DNA needs to be stored for a long time, it can be eluted with TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0), but EDTA may affect the downstream digestion reaction and may be appropriately diluted when used.
Downstream applications: DNA recovered using this kit can be used for a variety of routine procedures, including digestion, PCR, library construction, Southern hybridization, and more.
Product storage:
• Precipitation and precipitation may occur when the binding solution or inhibitor removal solution is cold. It can be re-dissolved in a water bath at 37 ° C for a few minutes. It is clear and transparent and then cooled to room temperature.
• To avoid reducing activity and facilitating transportation, proteinase K is provided as a lyophilized powder. After receiving, it can be centrifuged briefly, and dissolved in 1 ml of sterile ddH2O. Because repeated freezing and thawing may reduce the enzyme activity, it is immediately dispensed according to the amount of each use (10/20 μl) and stored at -20 °C.
• Avoid prolonged exposure to air, volatilization, oxidation, and pH changes. After use, tighten the lid after use.